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Thin-layer chromatography (2. B-mercaptoethanol (BME) A small molecule with free sulfhydryl groups often used to break disulfide bonds in proteins denaturant Chemical compound that destroys the 3D structure of proteins, especially by breaking hydrogen bonds guanidine Non-ionized form of guanidinium guanidinium chloride A widely used denaturant of proteins urea A nitrogen waste product of animals; also widely used as a denaturant of proteins 196 CHAPTER SEVEN Protein Structure and Function FIGURE 7.
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